practical virology from scratch
practical virology from scratch
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Published 4/2025
MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz, 2 Ch
Language: English | Duration: 2h 47m | Size: 2.14 GB
Practical Virology: From Basics to Lab Techniques
What you'll learn
ab construction and design
methods of viral diagnosis
viral cultivation in embryonated chicken egg
tissue culture techniques
Embryonated chicken egg
Requirements
biology knowledge - Understanding of cells, DNA/RNA, and basic microbiology concepts.
Interest in laboratory work - Since it's practical virology, being comfortable with the idea of handling biological materials is helpful.
Safety awareness - Some basic knowledge or willingness to learn lab safety protocols.
Description
Practical Virology from Scratch: A Beginner's GuideBy the end of the course, students should have a solid understanding of how viruses function, how they can be studied, and their importance in global health and biotechnology. Hands-on lab components and case studies will give practical experience to complement the theoretical aspectsVirology is the study of viruses-tiny microorganisms that can infect living organisms. Learning virology from scratch involves understanding the basic structure of viruses, how they interact with their hosts, and how we can study them safely in a lab.1. What are Viruses?Definition: Viruses are microscopic infectious agents made up of genetic material (DNA or RNA) enclosed in a protein coat, called a capsid. Some viruses also have an outer lipid envelope.Living or Non-living? Viruses are unique because they can't reproduce or carry out metabolism on their own. They need a host cell (like a human or animal cell) to multiply.2. Structure of VirusesCapsid: A protective protein shell that surrounds the virus's genetic material. The shape of the capsid can vary (helical, icosahedral, etc.).Genetic Material: Viruses can have either DNA or RNA, and this determines how they replicate in host cells.Envelope (optional): Some viruses, like HIV or influenza, have an outer lipid envelope that they "steal" from the host cell's membrane when they leave the cell.3. How Viruses WorkViruses infect by entering a host cell and hijacking its machinery to make more viruses. Here's the general process:Attachment: The virus attaches to the host cell using specific receptors.Entry: The virus enters the cell, sometimes by fusing with the cell membrane or through endocytosis (a process where the cell engulfs the virus).Replication: The virus's genetic material takes over the host's cellular machinery to replicate and produce new virus particles.Assembly & Release: New virus particles are assembled and then released from the host cell, either by budding (for enveloped viruses) or by causing the host cell to burst open (lysis).4. Studying Viruses in the LabIf you're interested in practical virology, you'll need to know how scientists study viruses:A. Lab Equipment and TechniquesCell Culture: Viruses can't grow outside of cells, so virologists grow them in cell cultures-living cells in a controlled environment. Different viruses need different types of cells.Plaque Assay: This method helps count the number of viruses. A virus infects a layer of cells, and each virus forms a plaque (a clear area where cells have been destroyed).PCR (Polymerase Chain Reaction): A technique used to detect viral genetic material by amplifying DNA or RNA, making it easier to study.Electron Microscopy: Since viruses are too small to see with normal microscopes, scientists use electron microscopes to see their structure.B. Biosafety Levels (BSL)Handling viruses in the lab requires strict safety protocols. Different viruses need different biosafety levels:BSL-1: Basic safety (e.g., non-infectious viruses).BSL-2: Moderate-risk pathogens (e.g., hepatitis, Zika).BSL-3: Higher-risk pathogens transmitted through the air (e.g., tuberculosis, SARS-CoV-2).BSL-4: High-risk, dangerous viruses like Ebola. Only a few labs in the world have this level.5. Key Concepts in VirologyViral Pathogenesis: How viruses cause disease in their hosts. Some viruses cause mild illness (like the common cold), while others cause severe diseases (like HIV or Ebola).Vaccine Development: Vaccines help train the immune system to recognize and fight viruses. Studying how viruses interact with the immune system is key to developing effective vaccines.Antiviral Treatments: Scientists also study how to develop drugs that stop viruses from replicating, like antiviral medications used to treat HIV or flu.6. Famous Viruses to KnowInfluenza Virus: Causes the flu, a contagious respiratory illness.Human Immunodeficiency Virus (HIV): A virus that attacks the immune system, causing AIDS.SARS-CoV-2: The virus responsible for the COVID-19 pandemic.Ebola Virus: A deadly vModule 1: Introduction to VirologyWhat Are Viruses?Definition and general characteristicsViruses vs. Bacteria and other microorganismsVirus life cycle overviewHistory of VirologyKey milestones in virologyImportant discoveries (e.g., tobacco mosaic virus, the discovery of bacteriophages)Virus ClassificationOverview of the classification systems (Baltimore classification, ICTV)DNA vs. RNA virusesEnveloped vs. non-enveloped virusesModule 2: Virus Structure and FunctionVirus MorphologyCapsid structures (helical, icosahedral, complex)Viral envelopes and spikes (glycoproteins)Overview of viral genome types (single-stranded, double-stranded, positive-sense, negative-sense)Viral Genome OrganizationTypes of viral genetic material (RNA, DNA)How genome structure impacts virus replicationVirus-Host InteractionsHow viruses attach to and enter host cellsMechanisms of viral replication inside host cellsAssembly and release of new viral particles (budding vs. lysis)Module 3: Methods in VirologyCell Culture TechniquesBasics of growing viruses in cell cultureDifferent types of host cells used for viral replicationSterile techniques for handling cell culturesViral Detection TechniquesPlaque assays to quantify viral particlesHemagglutination assaysEnzyme-Linked Immunosorbent Assay (ELISA)Polymerase Chain Reaction (PCR) for viral genome detectionAdvanced Virological ToolsUse of electron microscopy for viral visualizationFlow cytometry in virologyCRISPR and gene editing in virology researchModule 4: Viral PathogenesisViral Entry and Spread in the HostRoutes of viral entry (respiratory, gastrointestinal, sexual, etc.)Tropism: How viruses target specific cell typesModes of viral spread (local vs. systemic infections)Mechanisms of Viral DiseaseCytopathic effects of virusesImmune evasion strategiesHost immune response to viral infections (innate and adaptive immunity)Viral Latency and PersistenceHow viruses establish latency (e.g., herpesviruses)Mechanisms of chronic viral infection (HIV, hepatitis B)Module 5: Virology in Public HealthEpidemiology of Viral InfectionsHow viruses spread in populationsFactors affecting viral transmissionZoonotic viruses and cross-species transmissionOutbreaks and PandemicsCase studies: SARS, H1N1, COVID-19, EbolaGlobal strategies for controlling viral outbreaksRole of the World Health Organization (WHO) and CDCVaccines and ImmunizationHow vaccines work (live attenuated, inactivated, mRNA vaccines)The history and development of vaccines (e.g., polio, flu, COVID-19)Herd immunity and vaccination strategiesModule 6: Antiviral Therapies and ResistanceAntiviral Drug MechanismsMechanisms of action for key antiviral drugs (e.g., reverse transcriptase inhibitors, protease inhibitors)Case studies: HIV treatments, influenza drugsChallenges in Antiviral Drug DevelopmentDrug resistance mechanismsChallenges of treating chronic viral infectionsNew Approaches in Antiviral ResearchGene therapy and RNA interferenceNanotechnology in antiviral treatmentsModule 7: Emerging Viruses and BiosecurityNew and Re-emerging VirusesOverview of emerging viral threats (e.g., Zika virus, Nipah virus)Factors contributing to the emergence of new viruses (globalization, climate change)Biosafety and BiosecurityOverview of biosafety levels (BSL-1 to BSL-4)Handling dangerous viruses in high-containment labsGlobal security concerns related to bioterrorismModule 8: Laboratory and Practical ApplicationsHands-on Lab Techniques in VirologyPerforming cell culture and viral propagationConducting plaque assays and viral quantificationMolecular Virology TechniquesCloning viral genomesUsing CRISPR/Cas9 for studying viral genesData Analysis in VirologyAnalyzing virological data (e.g., viral growth curves)Using bioinformatics for viral genome sequencingModule 9: Case Studies and Research ApplicationsFamous Virology Case StudiesThe discovery of HIV and AIDS researchEradication of smallpox and lessons learnedStudying coronaviruses: From SARS to COVID-19Current Research in VirologyResearch on antiviral vaccines (e.g., mRNA vaccines)The role of virology in cancer research (oncoviruses)Module 10: Future of VirologyFrontiers in VirologyViral gene therapyVirus-based nanomedicineUse of viruses in biotechnology (e.g., bacteriophages in fighting antibiotic resistance)Preparing for Future PandemicsLessons from COVID-19Global collaborations and surveillance for future viral outbreaks
Who this course is for
Beginners in biology or life sciences
Pre-med or medical students - Those wanting hands-on virology experience
Lab techs or researchers - Looking to upskill or cross-train in virology techniques.
Biotech or pharma professionals - Needing foundational knowledge of viruses for work.
Homepage:
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https://www.udemy.com/course/practical-virology-from-scratch/
Download link
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https://rapidgator.net/file/eea0b3ebdede3ad3f575ad790b373f04/pruen.practical.virology.from.scratch.part3.rar.html
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https://nitroflare.com/view/D477849EE904AA4/pruen.practical.virology.from.scratch.part1.rar
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